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目的观察并比较正常人群与胃病患者口腔携带白色念珠菌菌株ITS基因型的差异情况。方法对162例普通人群和104例有不适症状就诊的胃病患者进行口腔念珠菌的采样、培养并鉴定,对分离培养出的白色念珠菌菌株进行内含子转录间隔区(ITS)测序,并建立进化树比对分析序列同源性,分析两组人群口腔携带的白色念珠菌菌株ITS基因型及其进化分支情况。结果正常人群口腔标本检测出白色念珠菌36株(36/162,22.2%),胃病患者口腔分离培养出白色念珠菌38株(38/104,36.5%),经ITS序列检测,正常人群18个菌株和胃病患者17个菌株申请Gen Bank序列注册号,两组人群携带菌株建立的进化树呈各自集中趋势。结论胃病患者口腔念珠菌携带率高于正常人口腔念珠菌携带率。正常人与胃病患者口腔中分离到ITS基因型进化关系不同的念珠菌菌株,且基因型具有多态性。
Objective To observe and compare the differences of ITS genotypes between oral and Candida albicans strains in normal population and stomach patients. Methods The Candida albicans samples were collected from 162 common people and 104 patients with gastropathy. The Candida albicans strains isolated and cultured were sequenced and sequenced. Phylogenetic tree analysis of sequence homology, analysis of oral Candida albicans ITS genotypes and their evolutionary branching in two groups of people. Results Candida albicans was detected in 36 (162 cases) and 162 (22.2%) of healthy volunteers in oral cavity. 38 (104%) and 36.5% (Candida albicans) were isolated from oral cavity of gastric patients. Seventeen strains of bacteria and gastritis patients applied for the Gen Bank sequence registration number. The phylogenetic trees established by the two groups of people carried their respective concentrated trends. Conclusion The oral candidiasis of gastric patients is higher than that of normal people. Candida strains with different ITS genotypes were isolated from the oral cavity of normal and gastric patients, and the genotypes were polymorphic.