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目的研究2型糖尿病患者CXCL5基因启动子-156位点G/C多态性分布,探讨该基因与糖尿病肾病的关系。方法应用多聚酶链式反应限制性片段长度多态性(PCR-RFLP)技术检测210例2型糖尿病患者﹝根据24h尿蛋白排泄率(UAER)分为DM组和DN组﹞及171例健康对照者趋化因子CXCL5基因启动子-156位点G/C多态性。结果 CXCL5基因启动子-156位点G/C多态性在对照组和2型糖尿病组中的分布差异无统计学意义(P﹥0.05);在DM和DN的患者中,CXCL5基因启动子-156位点GG基因频率与GC+CC基因频率相比差异有统计学意义(χ2=51.99,P﹤0.05)。两组G与C等位基因频率差异有统计学意义(χ2=54.72,P=0.000)。结论 CXCL5基因启动子-156位点G/C与中国河北秦皇岛地区汉族2型糖尿病发病无关,但其中C基因可能是2型糖尿病患者进展为DN的遗传学风险。
Objective To investigate the distribution of CXCL5 gene promoter-156 G / C polymorphism in patients with type 2 diabetes mellitus (T2DM) and to explore its relationship with diabetic nephropathy. Methods 210 patients with type 2 diabetes mellitus (divided into DM group and DN group according to 24h urinary protein excretion rate (UAER)) and 171 healthy controls were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Chemokine CXCL5 gene promoter-156 site G / C polymorphism. Results There was no significant difference in the distribution of CXCL5 gene promoter-156 G / C polymorphism between control group and type 2 diabetes mellitus (P> 0.05). Among the patients with DM and DN, CXCL5 gene promoter- The frequency of GG gene at 156 locus was significantly different from that of GC + CC gene (χ2 = 51.99, P <0.05). There was a significant difference in the frequencies of G and C alleles between two groups (χ2 = 54.72, P = 0.000). Conclusion The CXCL5 gene promoter-156 G / C is not associated with the onset of type 2 diabetes in Han nationality in Qinhuangdao of Hebei Province, China. However, the C gene may be the genetic risk of progression to DN in type 2 diabetic patients.