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在试验的两种Hm-毒素生物测定法中,根冠细胞测定法的效果比种子根伸长法好,主要是前者对Hm-毒素的敏感性强,与病斑大小有极显著的相关性。抑制种子根伸长法由于不能确切反映玉米对小斑病的反应,因而不宜用作Hm-毒素的生物测定。在不同的毒素制剂中,以病叶提取液处理根冠细胞最能反映小斑菌接种玉米后的实际情况,与病斑大小的相关系数r=0.9315,居供试的三种制剂之首,其次是粗提制剂和培养物滤液。试验还发现酒石酸铵不仅影响Hm-毒素测定的效果,而且还影响毒素产生的量,因而进行Hm-毒素的生物测定,最好使用病叶提取液或用无酒石酸铵的改良Fries培养基制备的毒素粗提制剂或培养物滤液。
Among the two tested Hm-toxin bioassays, the effect of root-crown cell assay was better than that of seed root elongation, mainly because the former was highly sensitive to Hm-toxin and had a very significant correlation with lesion size . Inhibition of seed-root elongation method can not accurately reflect the response of small spot disease of corn, and therefore should not be used as Hm-toxin bioassay. In different toxin preparations, the treatment of root and shoot cells with diseased leaf extract can best reflect the actual situation after inoculation with corn, and the correlation coefficient with lesion size was 0.9315, ranking the first of the three preparations tested, Followed by crude extract formulation and culture filtrate. It was also found that ammonium tartrate not only affected the effect of the Hm-toxin assay but also the amount of toxin produced, thus making a bioassay of Hm-toxin, preferably using a diseased leaf extract or a modified Fries medium without ammonium tartrate Toxins Crude extract or culture filtrate.