论文部分内容阅读
采用RT-PCR和原位杂交技术对6组分别代表不同生长发育时期的人卵巢组织中BRCA1 mRNA的表达进行了半定量分析和比较,同时与血清中的雌二醇(E_2)水平进行相关分析.结果BRCA1 mRNA的表达以早孕组、晚孕组和胎儿组较高(比正常生育期妇女高25%～75%),正常生育期妇女组其次,老年组和幼女组较低(约为正常生育期妇女的60%左右);BRCA1 mRNA的表达与血清E_2水平呈明显的正相关,相关系数为0.85.
The expression of BRCA1 mRNA in 6 groups of human ovarian tissues, which represent different growth and development stages, respectively, was semi-quantitatively analyzed and compared by RT-PCR and in situ hybridization. Correlation analysis was also performed with the level of serum estradiol (E 2) Results The expression of BRCA1 mRNA in early pregnancy group, late pregnancy group and fetus group was higher than that in normal pregnant women (25% -75% higher than that in normal pregnant women), followed by normal pregnant women group, followed by elderly group and young girl group About 60% of women in reproductive age). The expression of BRCA1 mRNA was positively correlated with serum E_2 level, with a correlation coefficient of 0.85.