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目的:探讨核转录因子-κB(NF-κB)、巨噬细胞迁移抑制因子(MIF)与子宫内膜异位症(EMS)的临床相关性。方法:采用逆转录聚合酶链反应(RT-PCR)法测定30例非EMS患者在位子宫内膜、68例EMS患者在位和异位子宫内膜的NF-κB和MIF mRNA水平。比较非EMS患者在位子宫内膜和EMS患者的在位子宫内膜及异位子宫内膜的NF-κB和MIF mRNA水平,并且分析EMS患者异位子宫内膜NF-KB、MIF mRNA水平与EMS分期的相关性。结果:①EMS患者异位子宫内膜和非EMS患者在位子宫内膜的NF-KB和MIF mRNA水平差异均具有统计学意义(P<0.01);②不同临床分期EMS患者异位子宫内膜NF-KB和MIF mRNA水平差异均具有统计学意义(P<0.01),并且随着临床分期的增加NF-KB和MIF mRNA水平依次增加(P<0.01);③相关性分析采用Spearman等级相关系数检验显示,EMS患者异位子宫内膜NF-KB与MIF mRNA水平、NF-KB水平与EMS分期、MIF mRNA水平与EMS分期均呈正相关(P<0.01)。结论:核转录因子-κB、巨噬细胞迁移抑制因子在EMS的发生、发展中起重要作用,有可能成为治疗EMS新的靶点。
Objective: To investigate the clinical relevance of nuclear factor-κB (NF-κB), macrophage migration inhibitory factor (MIF) and endometriosis (EMS). Methods: The levels of NF-κB and MIF mRNA in eutopic and ectopic endometrium of 30 patients with eutopic endometrium and 68 patients with EMS were determined by RT-PCR. The levels of NF-κB and MIF mRNA in eutopic endometrium and ectopic endometrium of EMS patients with eutopic endometrium and ectopic endometrium were compared, and the levels of NF-κB and MIF mRNA in ectopic endometrium of EMS patients were compared with those of EMS patients Correlation of EMS staging. Results: ①The levels of NF-κB and MIF mRNA in eutopic and ectopic endometrium of patients with EMS were significantly different (P <0.01); ② The ectopic endometrial NF (P <0.01), and the levels of NF-KB and MIF mRNA increased with the increase of clinical stage (P <0.01); ③The correlation analysis was carried out by Spearman’s rank correlation coefficient test The levels of NF-KB and MIF mRNA in ectopic endometrium of patients with EMS were positively correlated with EMS stage, MIF mRNA level and EMS stage (P <0.01). Conclusion: Nuclear factor-κB and macrophage migration inhibitory factor play an important role in the occurrence and development of EMS and may become a new target for the treatment of EMS.