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目的探讨多鼠MRI(multiple-mouse MRI,MMMRI)评价内皮抑素对结肠癌皮下移植瘤小鼠模型疗效的价值。方法建立小鼠结肠癌皮下移植瘤模型,1周后筛选成瘤均匀的16只随机分成内皮抑素组和生理盐水组,分别给予内皮抑素6mg/(kg.d)和0.9%生理盐水各0.2ml皮下注射,14d后行MMMRI检查,后行病理学检查。结果T1WI显示生理盐水组小鼠皮下脂肪层较薄或消失,瘤周脂肪层中断;内皮抑素组小鼠皮下脂肪层较厚。T2WI肿瘤呈不均匀高信号,部分肿瘤内见斑点状坏死区。MRI和病理测量的肿瘤体积分别为(2723.26±1136.91)mm3和(3505.76±1350.12)mm3,2种测量方法结果无统计学差异,且具有较高的相关性(r=0.993,P<0.01)。T1WI和T2WI2组肿瘤的绝对信号强度(ASI)无统计学差异。T1WI2组肿瘤的信号强度比(SIR)无统计学差异,而T2WI内皮抑素组肿瘤的SIR(3.19±0.28)较生理盐水组(2.60±0.47)高。结论MMMRI能准确、无创性反映内皮抑素对小鼠结肠癌皮下移植瘤的抑制作用。
Objective To investigate the value of multiple-mouse MRI (MMMRI) in evaluating the efficacy of endostatin in the mouse model of colon cancer xenograft. Methods A subcutaneous xenograft model of colon cancer was established in mice. One week later, 16 tumor-forming homogenates were randomly divided into endostatin group and normal saline group. Endostatin (6mg / (kg · d)) and 0.9% saline 0.2ml subcutaneous injection, 14d after MMMRI examination, after pathological examination. Results T1WI showed that the subcutaneous fat layer in mice in normal saline group was thin or disappeared, and the peritumoral fat layer was interrupted. The subcutaneous fat layer in endostatin group was thicker. T2WI tumors showed uneven high signal, see part of the tumor spotted necrosis. The volume of tumors measured by MRI and pathology were (2723.26 ± 1136.91) mm3 and (3505.76 ± 1350.12) mm3, respectively. There was no significant difference between the two methods (r = 0.993, P <0.01). There was no significant difference in absolute signal intensity (ASI) between T1WI and T2WI2 tumors. There was no significant difference in the signal intensity ratio (SIR) between T1WI2 tumors and T2WI endostatin tumors (3.19 ± 0.28) compared with saline group (2.60 ± 0.47). Conclusion MMMRI can accurately and noninvasively reflect the inhibitory effect of endostatin on subcutaneously transplanted colon cancer in mice.