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目的:探讨大豆异黄酮(Soybean Isoflavone,SIF)对子宫内膜异位症(endometriosis,EMs)大鼠模型中CD34和芳香化酶表达的影响。方法:采用外科手术法建立大鼠EMs模型,3周后测量移植物体积。EMs大鼠随机分为空白模型组(Ⅰ组)、SIF低剂量组(Ⅱ组,0.5 mg·kg-1·d-1),SIF高剂量组(Ⅲ组,50 mg·kg-1·d-1),造模成功后连续皮下注射药物12周,再次检测移植物体积变化,HE染色观察移植内膜的病理改变,免疫组织化学SABC法检测大鼠异位内膜组织中CD34及芳香化酶的表达。结果:该实验大鼠子宫内膜异位模型的形成率为76.4%。Ⅱ组治疗前、后移植物体积无明显差异(P>0.05),Ⅲ组治疗后移植物体积较治疗前明显减小,且较治疗后的其他组相比也明显减小,差异均有统计学意义(P<0.01)。Ⅱ组和Ⅲ组腺体中CD34和芳香化酶阳性表达率较Ⅰ组降低(P<0.05),且Ⅲ组中CD34和芳香化酶阳性表达率较Ⅰ组和Ⅱ组明显降低(P<0.01);子宫内膜异位症异位组织中芳香化酶及CD34的表达呈正相关(r=0.782,P<0.01)。结论:SIF对大鼠子宫内膜异位移植物有明显的退化作用,其机制可能与SIF通过降低CD34和芳香化酶表达来减弱内膜腺体及间质的迁移生长有关。
Objective: To investigate the effect of Soybean Isoflavone (SIF) on the expression of CD34 and aromatase in a rat model of endometriosis (EMs). Methods: The rat model of EMs was established by surgery. The volume of the graft was measured after 3 weeks. EMs rats were randomly divided into blank group (groupⅠ), SIF low dose group (groupⅡ, 0.5 mg · kg -1 · d -1), SIF high dose group (group Ⅲ, 50 mg · kg -1 · d -1). After successful modeling, the rats were injected subcutaneously for 12 weeks. The volume of the graft was measured again. The pathological changes of the intima were observed by HE staining. The expression of CD34 and aromatis in the ectopic endometrium were detected by immunohistochemical SABC method. Enzyme expression. Results: The formation rate of endometriosis model in this experimental rat was 76.4%. There was no significant difference between the two groups (P> 0.05) before and after treatment, and the volume of graft in group Ⅲ was significantly decreased after treatment compared with that before treatment, and also significantly decreased compared with other groups after treatment Significance (P <0.01). The positive rates of CD34 and aromatase in glands of group Ⅱ and group Ⅲ were lower than those of group Ⅰ (P <0.05), and the positive rates of CD34 and aromatase in group Ⅲ were significantly lower than those in group Ⅰ and group Ⅱ (P <0.01) ). The expression of aromatase and CD34 in ectopic endometriosis was positively correlated (r = 0.782, P <0.01). CONCLUSIONS: SIF has a marked degenerative effect on endometriotic xenografts in rats, and its mechanism may be related to SIF attenuating the migration and growth of the endometrial glands and stroma by reducing the expression of CD34 and aromatase.