诺如病毒(norovirus,NV)属人类杯状病毒科诺如病毒属,为无包膜单股正链RNA病毒,是引起人类急性胃肠炎散发和暴发的最主要病原体,也是食源性疾病的重要病原体,传播途径为传染源→粪便→水体或食品→人。目前,NV没有合适的组织细胞培养系统,在实验室诊断方面存在一定困难。过去主要采用电镜检测NV,然而,电镜检测灵敏度低,要求样本中病 Norovirus (NV) belongs to the human calicivirus Coronavirus, which is a non-enveloped single-stranded positive-sense RNA virus and is the most important causative agent of human acute gastroenteritis excretion and outbreak. It is also a foodborne disease Of the major pathogens, the source of infection for transmission → faeces → water or food → people. At present, NV does not have a suitable tissue culture system and there are some difficulties in laboratory diagnosis. In the past mainly used electron microscopy NV, however, the low sensitivity of electron microscopy, requiring samples of disease