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目的研究羟基喜树碱（ＨＣＰＴ）诱导胃癌细胞的凋亡作用，探讨其治疗胃癌的作用机制。方法应用ＭＴＴ、ＴＵＮＥＬ染色、流式细胞仪技术研究ＨＣＰＴ对胃癌细胞ＳＧＣ－７９０１、ＭＫＮ－４５、ＭＫＮ－２８的细胞毒和诱导凋亡的作用。结果ＨＣＰＴ具有很强的细胞毒作用，对三株不同分化程度胃癌细胞的ＳＧＣ－７９０１（中分化）、ＭＫＮ－４５（低分化）、ＭＫＮ－２８（６高分化）的ＩＣ５０为０．００８～０．０１２ｍｇ／ｍｌ。ＨＣＰＴ作用于细胞后，可看到较为典型的细胞凋亡的形态学变化：细胞核固缩，染色质凝集，呈新月型紧贴于核膜周边，核碎裂，染色质片断化，凋亡小体形成等。流式细胞仪ＤＮＡ直方图上出现典型的亚二倍体“凋亡峰”。流式细胞仪计数显示，ＨＣＰＴ诱导胃癌细胞ＳＧＣ－７９０１、ＭＫＮ－４５、ＭＫＮ－２８的凋亡率分别为２１．８８％、１２．３５％、３０．２６％。ＨＣＰＴ诱导胃癌细胞的凋亡率与胃癌的分化程度有关。ＴＤＴ染色法显示，细胞凋亡指数在１２．３５％～３０．２６％之间。ＨＣＰＴ的作用表现为细胞周期特异性，ＨＣＰＴ主要作用于细胞周期的Ｓ期，抑制细胞增殖和诱导细胞凋亡，使细胞阻滞于Ｓ期。结论ＨＣＰＴ对胃癌细胞具有很强的细胞毒作用，诱导凋?
Objective To study the apoptosis of gastric cancer cells induced by HCPT and explore its mechanism of action. Methods MTT, TUNEL staining and flow cytometry were used to study the effect of HCPT on cytotoxicity and apoptosis of gastric cancer cells SGC-7901, MKN-45 and MKN-28. Results HCPT had a strong cytotoxic effect. The IC50 of SGC-7901 (medium differentiation), MKN-45 (low differentiation), and MKN-28 (6 highly differentiated) of three different differentiation degree gastric cancer cells was 0.008- 0.012mg/ml. After HCPT acts on cells, we can see the typical morphological changes of cell apoptosis: nuclear condensation, chromatin condensation, crescent-shaped close to the nuclear membrane periphery, nuclear fragmentation, chromatin fragmentation, apoptosis Small body formation and so on. A typical hypodiploid “apoptotic peak” appears on the flow cytometer DNA histogram. Flow cytometry showed that the apoptosis rates of gastric cancer cells SGC-7901, MKN-45 and MKN-28 induced by HCPT were 21.88%, 12.35% and 30.26%, respectively. The apoptosis rate of gastric cancer cells induced by HCPT is related to the degree of differentiation of gastric cancer. TDT staining showed that the apoptosis index was between 12.35% and 30.26%. The role of HCPT is cell cycle specific. HCPT mainly acts on the S phase of cell cycle, inhibits cell proliferation and induces apoptosis, and arrests cells in S phase. Conclusion HCPT has a strong cytotoxic effect on gastric cancer cells and induces apoptosis.