分析基因表达的差异不仅在研究发育、分化、突变等领域有着极大的应用价值 ,已成为基因克隆非常有效的手段之一。cDNA代表性差异分析 (cDNARDA)是新近出现的一种新的、具有一定优越性的mRNA差异表达分析方法 ,本文简要介绍了cDNARDA的原理、方法 ,以及它和其它几种常用的mRNA差异表达分析技术如DD PCR、消减杂交、抑制消减杂交等进行了比较 Analysis of gene expression differences not only in the development, differentiation, mutation and other fields has a great value, has become a very effective means of gene cloning. cDNA Representative Differentiation Analysis (cDNARDA) is a new and emerging method of differential expression analysis of mRNA. This article briefly introduced the principle and method of cDNARDA and several other commonly used mRNA differential expression analysis Techniques such as DD PCR, subtractive hybridization, suppression subtractive hybridization were compared