论文部分内容阅读
为了探讨利用黄瓜毛状根来修复重金属镉(Cd)污染的可能性,研究了重金属Cd单独及其与锌(Zn)组合对黄瓜毛状根生长及其抗氧化酶SOD、POD活性变化的影响。结果表明,Cd≤10mg/L仅在培养5～15d间促进黄瓜毛状根生长,使根增粗;而Cd≥15mg/L则抑制黄瓜毛状根生长,浓度愈高抑制作用愈明显,侧根变得短而细小。在供试的不同浓度Cd培养的黄瓜毛状根中,除10mg/LCd外,其余Cd浓度培养的黄瓜毛状根可溶性蛋白含量随着培养时间的延长而逐渐下降;但其POD和SOD活性则随着培养时间的延长而逐渐升高。与对照(仅添加25mg/LZn)相比,仅1mg/LCd+25mg/LZn组合在培养7～15d期间促进黄瓜毛状根生长;其余浓度Cd和25mg/LZn组合都抑制黄瓜毛状根的生长,且Cd浓度愈高抑制作用越强,侧根数目更少且短小,侧根根尖变得肿胀;同时,除培养5d外,25mg/LZn和不同浓度Cd组合培养的黄瓜毛状根的生物量、POD和SOD活性均比单独添加对应浓度Cd培养的毛状根降低,但其可溶性蛋白含量则较之明显提高。结果表明:黄瓜毛状根具有较强的重金属Cd耐受能力,高浓度Cd则抑制其生长;而镉和锌组合会随着培养时间的延长而加重Cd对黄瓜毛状根生长的抑制作用。
In order to investigate the possibility of using cucumber hairy roots to repair heavy metal cadmium (Cd) pollution, the effects of heavy metal Cd alone or in combination with Zn on the growth of cucumber hairy roots and the changes of their antioxidant enzymes SOD and POD activities were studied. The results showed that the root growth of cucumber was promoted only when Cd≤10mg / L for 5 ~ 15d, while the root growth was curtailed when Cd≥15mg / L, the inhibition was more obvious when the concentration was higher, Become short and small. In the hairy roots of cucumber cultivated with different concentrations of Cd, the content of soluble protein in the hairy roots of the cucumber cultivated under the Cd concentration increased gradually with the increase of culturing time except the concentration of 10mg / L Cd. However, the activities of POD and SOD With the extension of incubation time and gradually increased. Compared with the control (only 25mg / L Zn added), only 1mg / L Cd + 25mg / L Zn combination promoted the hairy root growth of cucumber during the 7th ~ 15th day of culture; the other Cd and 25mg / LZn inhibited the growth of hairy root , And the higher the concentration of Cd was, the stronger the inhibition was, the number of lateral roots was smaller and shorter, and the root of lateral root became swollen. Meanwhile, the biomass of cucumber hairy roots incubated with 25mg / L Zn and Cd at different concentrations for 5d, Both POD and SOD activities were lower than that of hairy roots cultured with Cd at the corresponding concentration alone, but their soluble protein contents were significantly increased. The results showed that the hairy roots of cucumber had strong tolerance ability to heavy metals Cd and high concentrations of Cd inhibited the growth of cucumber roots. However, the combination of Cd and Zn increased the inhibitory effect of Cd on the growth of hairy roots.