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进一步确定SjR47和SjR12组分抗原的分子基础和性质及其SjR47免疫小鼠后的抗病变免疫效应.方法用SDS?PAGE电泳和酶联免疫印迹试验(ELIB)鉴定SjR47和SjR12两种组分抗原的分子基础和特性;用凝胶大柱制备电泳大量纯化、收集SjR47组分抗原并免疫BALB/c小鼠,攻击感染后6~8wk,取肝组织计算虫卵数并观察肉芽肿病变大小.结果进一步证明从日本血吸虫感染兔红细胞上分离、纯化的47kDa和12kDa组分抗原,前者主要属卵源性抗原,后者主要为虫源性抗原.用纯种小鼠进行抗病免疫实验结果表明,在感染后6~8Wk时,SjR47免疫小鼠肝内总减卵率和成熟卵减少率分别为52.57％~57.25％和50.73％~74.70％;肝内虫卵肉芽肿病变平均体积(101.57士11.51~87.95士19.55,1X105um3)显著小于佐剂对照组(143.54+10.77~110.96+15.34)及感染对照组(173.31+37.24~124.40+54.82)(P均“,”Aim Further demonstrated molecular basis and characteristics of SjR47 and SjR12 fractionalantigens,and studied on the anti--pathology effect of mice immunized wih SjR47. Method Molecular basis andcharacteristics of SjR47 and SiR12 fractional antigens were analysed by SDS--PAGE and enzymelinkedim-munoblotting techniques(ELIB). SjR47 was purified and colleted by a big PAGE column. BALB/c mice of wereimmunized with SjR47. From the 6th to the 8th week after infection eggs in the liver tissues of mice werecounted and egg granulorna formations were observed. Results It demonstrated that SjR47 and SjR12 fractional anti-gens which were separated from the erythrocytes of rabbits infected with Schistosoma japonicum shared egg originand worm origin respectively. The reduction rates of total eggs and mature eggs in the liver of SjR47--immunizedmice were 52. 57--57. 25 % and 50. 33--74. 70% respectively during the 6th to 8th week after infection,and thegranuloma mean volumes (1 X 105um3 )were 101. 57 + 11. 51 and 87. 95 + 19. 55 smaller than that (143. 54 + 10. 77and 110. 96 + 15. 34 ) of adjuvant control mice as well as that (173. 31 + 37. 24 and 124. 40 + 54. 82) of infectioncontrol mice (P<0. 01 ). Conclusion These results were similar to the previous findings and further demonstrat-ed that SjR47 would induce a strong anti--pathology immunity.