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采用高效液相和分光光度法对紫茎泽兰不同发育阶段(种子,芽,幼苗和成株)糖、激素、单宁及总黄酮含量差异进行了研究。结果表明:不同糖类的变化。紫茎泽兰的葡萄糖含量以芽最高,为6.93%;而种子(0.16%)、幼苗(1.27%)、成株(1.07%)均明显低于芽阶段;同样,紫茎泽兰中蔗糖含量芽较高,为2.68%,而种子(0.32%)、幼苗(0.52%)、成株(0.09%)均明显低于芽阶段。果糖含量的变化,最大值也是在芽阶段(3.28%),其次为种子阶段(2.56%),而种子和成株阶段均很低(0.14%和0.55%)。测定中,海藻糖、棉子糖、甘露糖的含量差别很大,仅种子阶段测定出3种糖的含量,结果表明差别不大,依次为0.04%、0.04%、0.03%。激素类的变化。在不同发育阶段,紫茎泽兰芽阶段吲哚乙酸(IAA)含量较高,达到14.20μg.g-1Dw,比种子阶段高1.25μg.g-1Dw,是幼苗的1.63倍,成株阶段的5.55倍。紫茎泽兰赤霉素(GA)含量在不同发育阶段存在着明显的差异,而且种子的GA含量(901.11μg.g-1Dw)明显高于芽、幼苗和成株阶段,而以芽和幼苗阶段含量最低,分别为97.35和84.29μg.g-1Dw,成株阶段略高于芽和幼苗阶段,为280.50μg.g-1Dw。单宁类的变化:紫茎泽兰单宁变化差异明显,紫茎泽兰种子中单宁含量为1.98%,明显高于芽、幼苗和成株阶段(<0.30%);总黄酮类的变化:紫茎泽兰总黄酮含量在发育过程中呈不断上升趋势。紫茎泽兰种子中总黄酮含量很微弱,为0.04%,而芽、幼苗和成株阶段的紫茎泽兰总黄酮含量均明显高于种子阶段,并且这3个阶段之间含量差别不大,分别为(0.60%、0.64%、0.62%)。这些结果说明,糖、激素、单宁及总黄酮含量与植物生长发育的基本规律和代谢过程的调控密切相关。
The contents of sugar, hormone, tannin and total flavonoids in different developmental stages (seed, shoot, seedling and plant) of Eupatorium adenophorum were studied by HPLC and spectrophotometry. The results show that: the changes of different carbohydrates. The highest content of glucose in shoots of Eupatorium adenophorum was 6.93%, while that of seeds (0.16%), seedlings (1.27%) and adult plants (1.07%) were significantly lower than bud stage The shoot height was 2.68%, while seed (0.32%), seedling (0.52%) and adult plant (0.09%) were significantly lower than bud stage. The maximum content of fructose in the bud stage (3.28%), followed by the seed stage (2.56%), while the seed and adult stage were very low (0.14% and 0.55%). The content of trehalose, raffinose and mannose varied greatly in the determination. The contents of three kinds of sugars were determined only in the seed stage. The results showed that the contents of trehalose, raffinose and mannose were not significantly different, followed by 0.04%, 0.04% and 0.03% respectively. Hormonal changes. At different developmental stages, IAA content of A. adenophora increased to 14.20μg.g-1Dw, 1.25μg.g-1Dw higher than that of seed stage, which was 1.63 times that of seedling, 5.55 times. The GA content in E. adenophorum was significantly different at different developmental stages, and the GA content (901.11μg.g-1Dw) in seeds was significantly higher than that in buds, seedlings and adult plants. However, The lowest was 97.35 and 84.29μg.g-1Dw respectively. The adult stage was slightly higher than the bud and seedling stage, which was 280.50μg.g-1Dw. The change of tannin: The difference of tannin of Eupatorium adenophorum is obvious. The content of tannin in Eupatorium adenophorum is 1.98%, which is obviously higher than that of bud, seedling and adult (<0.30%). The change of total flavonoids: purple The content of total flavonoids in Pueraria lobata increased in the process of development. The content of total flavonoids in Eupatorium adenophorum seeds was very weak, 0.04%, while the content of total flavonoids in Eupatorium adenophorum at bud, seedling and adult stage was significantly higher than that in seed stage, and the contents of these three stages were not significantly different , Respectively (0.60%, 0.64%, 0.62%). These results indicate that the content of sugar, hormone, tannin and total flavonoids are closely related to the basic rules of plant growth and development and the regulation of metabolic process.