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目的探讨姜黄素对甲醛致细胞氧化损伤的拮抗效应。方法采用A549细胞株作为实验材料,实验设对照组、0.1 mmol/L甲醛染毒组,姜黄素组(0.1 mmol/L甲醛+2.5～20.0 mg/L姜黄素),检测A549细胞中一氧化氮合酶(NOS),丙二醛(MDA),超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px)活性。结果甲醛染毒组A549细胞SOD、NOS和GSH-Px活性分别为(21.79±1.13)、(1.88±0.16)与(27.83±0.2)U/mgprot,与对照组比较,SOD、NOS和GSH-Px活性明显下降(P<0.05),MDA含量[(3.87±0.153.87)nmol/mgprot]明显升高(P<0.05)。与甲醛染毒组比较,各姜黄素组GSH-Px活性上升、MDA含量下降(P<0.05),与对照组比较,40 mg/L姜黄素组GSH-Px活性、MDA含量差异无统计学意义(P>0.05)。结论姜黄素可提高A549细胞抗氧化酶活性,并存在剂量效应关系。
Objective To investigate the antagonistic effect of curcumin on formaldehyde-induced oxidative damage in cells. Methods A549 cells were used as experimental materials. The control group, 0.1 mmol / L formaldehyde exposure group and curcumin group (0.1 mmol / L formaldehyde + 2.5-20.0 mg / L curcumin) (NOS), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity were measured. Results The SOD, NOS and GSH-Px activities of formaldehyde-exposed A549 cells were (21.79 ± 1.13), (1.88 ± 0.16) and (27.83 ± 0.2) U / mgprot, (P <0.05), MDA content [(3.87 ± 0.153.87) nmol / mgprot] increased significantly (P <0.05). Compared with the control group, GSH-Px activity and MDA content in each curcumin group decreased (P <0.05). Compared with the control group, GSH-Px activity and MDA content in 40 mg / L curcumin group showed no significant difference (P> 0.05). Conclusion Curcumin can increase the anti-oxidative enzyme activity of A549 cells, and there is a dose-response relationship.