[目的]优化柞蚕Antheraea pernyi基因组注释,更好地扩展其在比较基因组学及品种改良研究中的应用.[方法]对柞蚕进行全长转录组测序分析;经全长转录本与参考基因组比对,鉴定新基因及新转录本,并对这些新基因和新转录本进行功能注释及长链非编码RNAs (lncRNAs)预测.利用大量的蛋白质编码转录本和lncRNAs对柞蚕基因组中基因结构进行修订.最后创建矫正后的柞蚕基因组基因注释.[结果]新发现1997个蛋白编码基因和3399个lncRNA基因,分别由2402个和3574个全长转录本数据支持.发现柞蚕基因组合25021个基因,其中19825个基因是蛋白编码基因,包括7个保幼激素酸甲基转移酶基因.[结论]本研究促进了对柞蚕基因组基因注释信息的认识,为柞蚕及相关物种功能基因组及比较基因组学研究提供了很有用的数据资源.“,”[Aim]This study aims to improve the annotation of the genome of the Chinese oak silkworm,Antheraea pernyi,so as to expand its application to comparative genomics and breed improvement.[Methods]The full-length transcriptome of A.pernyi was sequenced and analyzed,and compared with the reference genome to identify novel genes and transcripts with functional annotation and to predict long non-coding RNAs(lncRNAs).The gene models in the genome of A.pernyi were modified by thousands of novel protein-coding transcripts and lncRNAs.Finally,the corrected and merged annotation of the genome of A.pernyi was created.[Results]A total of 1997 novel protein-coding genes and 3399 novel lncRNA genes were discovered and supported by 2402 and 3574 full-length transcripts,respectively.The genome of A.pernyi contains 25021 genes,including 19825 protein-coding genes,from which seven juvenile hormone acid O-methyltransferase genes were identified.[Conclusion]This study improves our knowledge of the known genes in the genome of A.pernyi and provides valuable resources for comparative and functional genomic studies in A.pernyi and its relatives.