This research was carried out in the area of experimental embryology, which covers from animal fertilization to cloning. Two achievements in the field of reproductive biology have created major influence in the past 50 years. One is the discovery of sperm capacitation and the establishment of in vitro fertilization technology, and the other is the birth of somatic cell nuclear transfer animals. Although researches in reproductive biology have developed very quickly and a lot of literatures have been published, many basic theoretical questions are still remained illusive. This paper gives a brief description of our researches during the past decade.