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本文旨在应用标准玻璃微电极技术,观察白藜芦醇对离体豚鼠乳头状肌的电生理效应。结果显示:(1)白藜芦醇(30、60、120 μmol/L)可剂量依赖性地缩短乳头状肌细胞的动作电位时程;(2)对部分去极化的乳头状肌,白藜芦醇(60 μmol/L)不仅缩短动作电位时程, 而且降低动作电位的幅值和超射值, 减慢零期最大上升速度;(3)用无钙K-H 液灌流标本可完全取消白藜芦醇对乳头状肌细胞的作用;(4)钾通道开放剂四乙基氯化铵(TEA, 20 mmol/L), 不能阻断白藜芦醇的电生理效应;(5)预先应用一氧化氮合酶抑制剂L-NAME(1 mmol/L), 对白藜芦醇的上述效应无影响。以上结果表明,白藜芦醇可缩短正常乳头状肌细胞动作电位时程,这一效应可能与其抑制钙离子内流有关,但此作用机制中 NO 的作用并不显著。
This article aims to use standard glass microelectrode technology to observe the electrophysiological effects of resveratrol on isolated guinea pig papillary muscles. The results showed that: (1) resveratrol (30, 60, 120 μmol/L) dose-dependently shortened the action potential duration of papillary muscle cells; (2) partially depolarized papillary muscles, white Resveratrol (60 μmol/L) not only shortens the duration of action potential, but also decreases the amplitude of action potential and overshoot, slowing down the maximum rise time of zero phase; (3) White perfusion with calcium-free KH solution can completely eliminate white Effects of resveratrol on papillary muscle cells; (4) Potassium channel opener tetraethylammonium chloride (TEA, 20 mmol/L) does not block the electrophysiological effects of resveratrol; (5) Pre-application The nitric oxide synthase inhibitor L-NAME (1 mmol/L) had no effect on the above effects of resveratrol. The above results show that resveratrol can shorten the action potential duration of normal papillary muscle cells. This effect may be related to its inhibition of calcium influx, but the role of NO in this mechanism of action is not significant.