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目的：：为探讨sipB和crp基因对侵袭能力的影响。方法：以鼠伤寒沙门菌SL1344株sipB和crp缺失株为研究对象，进行生物学特性以及体内外侵袭力的研究。结果：生物学特性结果表明，sipB缺失株生化特性和亲本菌株相比没有发生明显的变化，而crp缺失株失去了利用麦芽糖、乳糖、山梨醇等碳源的能力，并且生长速度较亲本菌株相比发生了显著的降低。体内侵袭力结果表明两个实验组小鼠肝脏和脾脏分离到的细菌数目均低于对照组，体外感染HeLa细胞结果显示sipB缺失菌株组的黏附率和侵入率较crp组和对照组均发生了显著的降低。结论：以上结果表明sipB基因在细菌侵染机体过程中扮演着重要的角色。“,”Objective:To investigate the effect of its invasion capacity after the sipB and crp gene deletion strains of Salmonella typhimurium SL1344.Methods:Salmonella typhimurium SL1344 ΔsipB and Δcrp biological characteristics and invasion capacity were studied .Results:The SL1344Δcrp mutant was not able to ferment maltose, lactose, and sorbitol.The growth rate of the mutant was more slowly than SL1344 strain.The dynamic distribution measurement results showed that the number of bacteria in the two experimental groups of mice were isolated from the liver and spleen were lower than in the SL1344 control group .Next HeLa cells infected results showed that lack of the adhesion rate and invasion rate of theΔsipB strain group was lower thanΔcrp strain group and the SL1344 control group .Conclusion: These results showed that the SipB protein play an important role in the process of bacteria infection the body.