论文部分内容阅读
Total proteins in the pollen of Humulus scandens Lour，one of the most popular aeroallergens in China，were analyzed by two-dimensional electrophoresis in the current study.The proteins were extracted by Trichloracetic acid (TCA) method，and then separated by isoelectric focusing as the first dimension and SDS-PAGE as the second dimension.The spots of proteins were visualized by staining with Coomassie Brilliant Blue.After analysis with software (ImageMaster 2D)，122 different proteins were detected;isoelectric point (pI)，Molecular weight (MW) and relativevolume of each protein in the pollen were also discovered.This is the first high-resolution，two-dimensional protein map of the pollen ofHumulus scandens Lour in China.Our finding has built a solid foundation for identification，characterization，gene cloning and standardization of allergenic proteins in the pollen ofHumulus scandens Lour for further studies.