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目的 观察肝门胆管癌患者外周血淋巴细胞染色体的不稳定性 ,探讨肝门胆管癌的遗传易感性 ;找寻外周血标记物 ,为肝门胆管癌临床的早期诊断提供依据。方法 用染色体显带分析技术和荧光原位杂交 (FISH)分析技术对 2 6例肝门胆管癌患者、2 6例正常人外周血淋巴细胞染色体不稳定性进行了观察。结果 肝门胆管癌患者外周血淋巴细胞染色体裂隙、断裂和异常细胞为 ( 8.75± 3 .3 0 )、( 7.3 6± 2 .76)、( 13 .3 5± 4.73 ) ,而正常对照组为 ( 3 .17± 1.82 )、( 2 .0 4± 1.76)、( 3 .65± 1.97) ,经t检验 ,患者组与对照组各检测指标差异均有显著性 (P <0 .0 1) ;频率较高的位点是 8p2 2、9p2 1、11q13、17p12。 结论 肝门胆管癌患者外周血淋巴细胞染色体裂隙、断裂和异常细胞比率明显增高 ,提示肝门胆管癌患者可能存在较高的肿瘤遗传易感性 ;位点 8p2 2、9p2 1、11q13、17p12可能是寻找肝门胆管癌外周血标记物的候选部位。
Objective To observe the chromosomal instability of peripheral blood lymphocytes in patients with hilar cholangiocarcinoma and to investigate the genetic susceptibility of hilar cholangiocarcinoma. To search for peripheral blood markers to provide evidence for the early clinical diagnosis of hilar cholangiocarcinoma. Methods The chromosomal instability of peripheral blood lymphocytes in 26 patients with hilar cholangiocarcinoma and 26 healthy controls were observed by chromosome banding analysis and fluorescence in situ hybridization (FISH). Results Chromosome cleft, rupture and abnormal cells of peripheral blood lymphocytes in patients with hilar cholangiocarcinoma were (8.75 ± 3.30), (7.36 ± 2.76) and (13.35 ± 4.73), respectively, while those in the control group were (3.17 ± 1.82), (2.04 ± 1.76) and (3.65 ± 1.97) respectively. There was significant difference between the test group and the control group by t-test (P <0.01) ; The more frequent sites were 8p2 2,9p2 1,11q13,17p12. Conclusions Hepatociliary cholangiocarcinoma patients with peripheral blood lymphocytes chromosome fissure, fracture and abnormal cell ratio was significantly increased, suggesting that patients with hilar cholangiocarcinoma may have a higher genetic susceptibility to cancer; site 8p2 2,9p2 1,11q13,17p12 may be Looking for candidate sites for hilar cholangiocarcinoma peripheral blood markers.