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目的 探讨催化信号扩增法 (catalyzedsignalampli fication ,CSA)在检测组织内微量抗原中的作用及意义。方法 分别应用免疫组织化学ABC法和CSA法 ,对 5 4例霍奇金淋巴瘤 (Hodgkin’slymphoma ,HL)和 1例人巨细胞病毒 (humancytomegalovirus,HCMV)感染的对照涎腺组织中 ,HCMV的立即早期抗原DDG9、早期抗原CCH2和低基质蛋白抗原AAC10进行检测 ,并比较阳性信号的强弱及阳性率。结果 应用ABC法 ,仅在 1例对照组织中检测到DDG9、CCH2及AAC10的弱阳性信号 ,在 5 4例HL中 ,未检测到任何阳性信号 ;而应用CSA法不仅对照组织中DDG9、CCH2及AAC10均呈强阳性 ,而且有 6例 (11.1% )HLDDG9/CCH2呈阳性 ,5例 (9.3% )HLAAC10呈阳性。结论 CSA是一种较ABC法敏感的免疫组织化学染色方法 ,可检测出ABC法未能检测出的组织内微量抗原
Objective To investigate the role and significance of catalyzed signal amplification (CSA) in the detection of trace antigens in tissues. Methods Immunohistochemical ABC method and CSA method were used to detect the expression of HCMV in 54 cases of Hodgkin’s lymphoma (HL) and 1 control human salivary gland infected with human cytomegalovirus (HCMV) Immediate early antigen DDG9, early antigen CCH2 and low matrix protein antigen AAC10 were detected, and the positive signal intensity and positive rate. Results ABC method was used to detect weak positive signals of DDG9, CCH2 and AAC10 in only one case of control. No positive signal was detected in 54 cases of HL. The CSA method not only detected DDG9, CCH2 and AAC10 were strongly positive, and 6 (11.1%) were positive for HLDDG9 / CCH2 and 5 (9.3%) were positive for HLA-A10. Conclusion CSA is a sensitive immunohistochemical staining method compared with ABC method and can detect trace antigens in tissue that can not be detected by ABC method