目的　考察百日咳、白喉、破伤风、乙肝四联疫苗的接种反应及血清学效果。方法　选择足月分娩、母亲乙肝表面抗原阴性、身体健康的新生儿为接种对象。一期观察 6 0例 ,随机分为 3组 ,第 1组按 2、4、6月龄接种四联疫苗 ;第 2组按 3、4、5月龄接种四联疫苗 ;第 3组按 3、4、5月龄接种三联疫苗。二期观察 35 0人 ,随机分为3组 ,第 1组按 2、4、6月龄接种四联疫苗 ,118人 ;第 2组按 2、4、6月龄左臂接种乙肝疫苗 ,右臂接种三联疫苗 ,114人 ;第 3组按 0、1、6月龄接种乙肝疫苗 ,3、4、5月龄接种三联疫苗 ,118人。结果　一期观察 1组、2组和 3组体温弱反应分别为 5 0 %、10 %和 2 0 % ,无中强反应和局部反应。二期观察试验组体温弱反应为 4 2 4 % ,中反应 1 6 9% ,无强反应。局部弱反应为 0 85 % ,无中、强反应。血清学检测百日咳、白喉、破伤风、乙肝阳转率均大于 90 %。结论　吸附四联疫苗具有较好的安全性 ,采用 2、4、6月龄接种程序可有效诱导产生百日咳、白喉、破伤风、乙肝的保护性抗体反应 Objective To investigate the vaccination and serological effects of whooping cough, diphtheria, tetanus and hepatitis B vaccine. Methods Select full-term delivery, the mother of hepatitis B surface antigen-negative, healthy newborn vaccination. A group of 60 cases, were randomly divided into three groups, the first group by 4, 6 months of age vaccination of four vaccine; group 2 by 3, 4, 5 months of age vaccination vaccine; group 3 by 3 , 4, 5 months old vaccination triple vaccine. In the second phase, 35 0 patients were randomly divided into 3 groups. In group 1, 4 vaccines were vaccinated at 2, 4 and 6 months, and 118 were vaccinated. In group 2, mice were vaccinated with left arm at 2, 4 and 6 months, Arm vaccinated triple vaccine, 114; group 3 by 0, 1, 6 months of age vaccination of hepatitis B vaccine, 3, 4, 5 months of age vaccination triple vaccine, 118 people. Results In the first phase, the weakness of body temperature in groups 1, 2 and 3 were 50%, 10% and 20% respectively. There was no moderate reaction and local reaction. Second phase observation test group body temperature weak reaction was 4 2 4%, medium reaction 1 6 9%, no strong reaction. Local weak reaction was 0 85%, no medium and strong reactions. Serological tests pertussis, diphtheria, tetanus, hepatitis B positive rate of more than 90%. Conclusions The four-in-four vaccine has good safety. The two, four and six months vaccination program can effectively induce the protective antibody response to pertussis, diphtheria, tetanus and hepatitis B