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对八角枫Alangium chinense根的化学成分进行研究,运用硅胶、凝胶、MCI-gel树脂及RP-HPLC等多种色谱技术分离纯化,并根据理化性质和波谱数据鉴定化合物的结构,同时用MTT法测定化合物的细胞毒活性。从八角枫根的90%乙醇提取物中分离鉴定了3个生物碱类化合物(1~3),其中化合物1为新化合物,命名为8-羟基-3-羟甲基-6,9-二甲基-7H-苯并[de]异喹啉-7-酮,其对NB4,A-549,SHSY5Y,PC-3,MCF-7的IC50分别为4.2,3.5,5.7,2.8,3.9μmol·L-1,该化合物具一定的细胞毒活性。
The chemical constituents of the roots of Alangium chinense were isolated and purified by silica gel, gel, MCI-gel resin and RP-HPLC. The structures of the compounds were identified by their physico-chemical properties and spectral data. At the same time, The cytotoxic activity of the compounds was determined. Three alkaloids (1 ~ 3) were isolated and identified from 90% ethanolic extracts of Chinese maple root. Compound 1 was a new compound named as 8-hydroxy-3-hydroxymethyl-6,9- Methyl-7H-benzo [de] isoquinolin-7-one having IC50 of 4.2, 3.5, 5.7, 2.8 and 3.9 μmol ·, respectively for NB4, A-549, SHSY5Y, PC-3 and MCF-7 L-1, the compound has a certain cytotoxic activity.