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从植物愈伤组织中定向筛选抗病突变体,国内多用三角瓶做为筛选皿。此法最困难的问题是把不耐高温的筛选压力快速加入培养基内,而且三角瓶多占空间、操作不便、洗涤麻烦,培养基用量也大。利用培养皿替代三角瓶,可大大提高筛选效率。方法是按不同试验要求把配好的基本培养基分装在不同大小的三角瓶中,使用时加热融化,待冷却到45℃左右,用灭菌移液管加入一定数量的激素和筛选压力,混匀,倒入消毒的培养皿内,每皿约10～15毫升;把愈伤组织
Targeted screening of disease-resistant mutants from plant callus, the domestic use of triangular flask as a screening dish. The most difficult problem of this method is that the pressure of heat-resistant screening is rapidly added to the culture medium, and the space occupied by the flask is inconvenient, the operation is inconvenient, and the amount of medium used is also large. The use of petri dishes instead of triangular flask, can greatly improve the screening efficiency. Method is based on different test requirements with a good basic medium sub-packed in triangular flask of different sizes, the use of heat to melt, to be cooled to about 45 ℃, with a sterile pipette to join a certain amount of hormones and screening pressure, Mix well, into sterile dish, each dish about 10 to 15 ml; the callus