Chronic human immunodeficiency virus(HIV)infection not only causes a gradual loss of CD4 T cells but also leads to a disturbance of the T cell receptor(TCR)repertoire.In people living with HIV(PLWH),monitoring TCR repertoire is challenged by the inconsistency of complementarity determining region 3(CDR3)and limited cell numbers in clinical samples.Thus,a quantitative method is necessary for monitoring the TCR repertoire in PLWH.We characterized the TCR V-J pairing profile of na?ve and memory CD4+T cells in healthy donors,HIV-infected antiretroviral therapy(ART)-naive patients and long-term(over 5 years)ART-experienced patients by performing TCR sequencing.We developed a V-J index with 18 parameters which were subdivided into five categories(expression coverage,cumulative percentage of the top tenth percentile,diversity,intra-in-dividual similarity and inter-individual similarity).In ART-na?ve patients,14 of the 18 parameters were significantly altered.Long-term ART recovered ten parameters.The four unrecovered parameters were related to inter-individual similarity.Therefore,these findings indicate that long-term ART could only partially recover TCR V-J pairs and introduce newly impacted V-J pairs.Moreover,these results provide new insights into the V-J pairing of the TCR and into the disturbance of TCR repertoire in HIV infection.