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目的 检测pEgr-Endostatin-EGFP质粒的表达特性和pEgr-Endostatin-EGFP基因-放射治疗小鼠黑色素瘤的作用.方法 pEgr-Endostatin-EGFP重组质粒用脂质体方法转染B16细胞,采用Westernblot方法检测Endostatin表达.建立小鼠荷瘤模型,注射质粒并给予5 Gy X射线照射,共3次,18天观察肿瘤生长情况,并采用免疫组织化学方法检测肿瘤血管生成.结果 转染了pEgr-Endostatin-EGFP质粒的B16细胞和转染并接受照射的B16细胞组可以检测到Endostatin蛋白表达.C57BL/6J小鼠给予pEgr-Endostatin-EGFP质粒注射并接受肿瘤X射线照射,可以显著抑制肿瘤生长(P<0.01),而且肿瘤局部血管生成明显降低(P<0.01).结论 本研究数据为肿瘤基因-放射治疗提供了新的治疗方案.“,”Objective To investigate the expression properties of pEgr-Endostatin-EGFP plasmid and the effects of pEgr-Endostatin-EGFP gene-radiotherapy on melanoma tumor-bearing mice. Methods The pEgr-En-dostatin-EGFP plasmid was transfected into B16 cell line with liposome. The expression property of Endostatin was investigated by western blot. Tumor-bearing mice were treated by the plasmid injection and then with 5 Gy X-irradiation for three times. Tumor growth was observed for 18 days after treatment. The formation and change of tumor capillary were measured with histoehemistry assay at the end of the experiment. Results The expression of Endostatin could be detected in B16 melanoma cells which transfected with pEgr-Endostatin-EGFP plasmid, no matter the cells received 2 Gy irradiation or not. The C57BL/6J mice, which have been treated with B16 melanoma, pEgr-Endostatin-EGFP recombinant plasmid and local X-irradiation, showed significant differ-ence in the inhibitory effect on the turnor growth (P<0.01) comparing with the control groups.. The inhibitory effect of pEgr-Endostatin-EGFP gene-radiotherapy on the tumor growth is correlated with the marked decrease of intraturnoral vascularization (P<0.01). Conclusion This work provides a potential antiangiogenic approach in gene-radiotherapy for cancer.