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目的:探讨保肝颗粒对四氯化碳(CCl4)诱导的实验性肝纤维化大鼠纤维化水平的影响。方法:大鼠实质损伤性纤维化模型由CCl4诱导。60只雄性Wister大鼠被随机平均分为6组:正常对照组、模型组、保肝颗粒高、中、低剂量组及马替洛酯组。除正常对照组外所有大鼠均给予皮下注射40%CCl4(每3天1次,共11周)。造模3周后同时分别给予保肝颗粒18、9.0、4.5g/(kg.d)及马洛替酯90mg/(kg.d),正常对照组给予等量生理盐水,每天1次,共8周。实验结束时检测肝功能、血清血清透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)和肝脏羟脯氨酸(HYP),采用光镜观察病理组织学改变。结果:保肝颗粒能改善肝纤维化大鼠的肝功能,高、中剂量可显著降低血清HA、LN、PCⅢ水平和肝组织中过高的HYP;病理组织学检查亦表明其能显著改善肝纤维化程度。结论:保肝颗粒对CCL4诱导的大鼠肝纤维化有良好的防治作用。
Objective: To investigate the effect of Baogan Granules on fibrosis in experimental liver fibrosis induced by carbon tetrachloride (CCl4). METHODS: Rat parenchymal fibrosis models were induced by CCl4. Sixty male Wister rats were randomly divided into six groups: normal control group, model group, high-, middle-, and low-dose groups of Baogan Granules and matelate. All rats except the normal control group were given subcutaneous injections of 40% CCl4 (once every 3 days for a total of 11 weeks). After 3 weeks of modeling, Baogan Granules of 18, 9.0, 4.5 g/(kg.d) and malotilate 90 mg/(kg.d) were given at the same time. The normal control group was given the same amount of physiological saline once a day for a total of 8 weeks. At the end of the experiment, liver function, serum serum hyaluronic acid (HA), laminin (LN), type III procollagen (PCIII), and liver hydroxyproline (HYP) were measured. Histopathological changes were observed by light microscopy. Results: Baogan granules can improve liver function in rats with hepatic fibrosis. High and middle doses can significantly reduce serum HA, LN, PC III levels and excessive HYP in liver tissues. Histopathological examination also showed that hepatic granules can significantly improve liver function. Degree of fibrosis. Conclusion: Baogan Granules have good preventive and therapeutic effects on CCL4-induced liver fibrosis in rats.