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淋巴细胞含有肾上腺糖皮质激素受体,能和多种肾上腺皮质激素结合,一般说来,结合能力越强,其临床作用也越强。实验用新鲜的人体完整的淋巴细胞与6种挑选的肾上腺皮质激素结合,以氚标记地塞米松作为受体结合的竞争物,来观察6种激素结合力的强弱,以供临床用药时的参考。 用新鲜血400ml,EDTA抗凝。以比重为1.077～1.080的Ficoll和Hypague混合液离心分离血小板,红细胞和多形核白细胞,收集淋巴细胞于Hank’s液中,混悬液含9～11×10~6/ml淋巴细胞。用煤焦
Lymphocytes contain glucocorticoid receptors, which bind to various adrenocortical hormones. In general, the stronger the binding capacity, the stronger their clinical utility. Experiments with fresh human intact lymphocytes and six kinds of selected adrenocorticotropic hormone binding to tritiated dexamethasone as a receptor binding competition to observe the strength of the six hormone binding for clinical use reference. With fresh blood 400ml, EDTA anticoagulant. The platelets, erythrocytes and polymorphonuclear leukocytes were isolated by centrifugation in a mixture of Ficoll and Hypague with a specific gravity of 1.077 ~ 1.080. The lymphocytes were collected in Hank’s solution containing 9 ~ 11 × 10 ~ 6 / ml lymphocytes. With char