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目的研究冬凌草甲素注射剂诱导人胃癌SGC-7901细胞凋亡及与细胞内Ca2+浓度([Ca2+]i)的关系。方法 MTT法检测冬凌草甲素注射剂对SGC-7901细胞的细胞毒活性;倒置显微镜观察冬凌草甲素注射剂对细胞形态学的影响;流式细胞术测定细胞凋亡率;激光共聚焦显微术检测SGC-7901细胞[Ca2+]i的变化;JC-1单染,激光共聚焦显微术检测SGC-7901细胞中线粒体膜电位的变化。结果冬凌草甲素注射剂对SGC-7901细胞的IC50为21.74μmol/L;作用48 h后,细胞生长密度变疏,细胞皱缩,其中冬凌草甲素注射剂高浓度组大部分细胞破碎。冬凌草甲素注射剂5.5、11、22μmol/L作用24 h后SGC-7901细胞凋亡率分别为7.07%、18.57%、22.96%;钙离子荧光强度高于对照组;线粒体膜电位荧光强度显著低于对照组。结论冬凌草甲素注射剂通过升高SGC-7901细胞[Ca2+]i诱导细胞凋亡。
Objective To investigate the relationship between the apoptosis of SGC-7901 cells and the intracellular Ca2 + concentration ([Ca2 +] i) induced by oridonin injection. Methods The cytotoxicity of oridonin injection to SGC-7901 cells was detected by MTT assay. The morphological changes of cells treated with oridonin A were observed by inverted microscope. The apoptosis rate was detected by flow cytometry. The changes of [Ca2 +] i in SGC-7901 cells were detected by micro-technique. The changes of mitochondrial membrane potential in SGC-7901 cells were detected by JC-1 single staining and confocal laser scanning microscopy. Results The results showed that the IC50 of oridonin injection to SGC-7901 cells was 21.74μmol / L. After 48 hours, the cell density was reduced and the cells were shrunk. Most cells in the high concentration of oridonin injection were broken. The apoptosis rates of SGC-7901 cells treated with oridonin A for 5.5 h at 5.5, 11 and 22 μmol / L were 7.07%, 18.57% and 22.96%, respectively. The fluorescence intensity of calcium ion was higher than that of the control group. The mitochondrial membrane potential fluorescence intensity was significant Lower than the control group. Conclusion Oridonin injection can induce apoptosis in SGC-7901 cells by increasing [Ca2 +] i.