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分析了高强纤维双价转基因抗虫杂交棉苏杂3号的品种特性,结果显示:①苏杂3号纤维强力高、品质优,HVICC纤维长度30.3 mm、比强度34.3 cN/tex、整齐度85.2%、伸长率7.1%、马克隆值4.6、反射率77%、黄度8.1、纺纱均匀性指数158;②苏杂3号综合抗病虫性能好,抗棉铃虫、高抗红铃虫、高耐枯萎病、耐黄萎病;③苏杂3号产量水平高,子棉单产3 359.4 kg/hm2,比对照泗棉3号增产15.14%;皮棉单产1 304.7 kg/hm2,比对照增产0.37%。苏杂3号集高强纤维、高产、抗虫于一体,是新一类型的高品质国产双价抗虫杂交棉。抗虫蛋白试剂条检测显示,苏杂3号具有B t毒蛋白的高阳性表达。PCR检测结果表明,苏杂3号含有中国合成构建的外源B t+CpTI双价杀虫基因片段。
The results showed that: ①Suza No.3 fiber had high strength and good quality, the length of HVICC fiber was 30.3 mm, the specific strength was 34.3 cN / tex, the uniformity was 85.2 %, Elongation 7.1%, micronaire value 4.6, reflectance 77%, yellowness 8.1, spinning uniformity index 158; ② Suza No.3 comprehensive pest resistance, resistance to bollworm, high resistance to pink bollworm, high Resistance to Verticillium wilt and Verticillium wilt resistance; ③The yield of Suzao 3 was high, yielding 3 359.4 kg / hm2 of seed cotton, 15.14% more than that of the control Simian 3; 1 304.7 kg / hm2 of lint yield increasing 0.37% . Su-3 set of high-strength fiber, high yield, insect resistance in one, is a new type of high-quality domestic double insect resistant hybrid cotton. Insect-resistant protein reagent strip test showed that Suza 3 has a high positive expression of B t toxin protein. The results of PCR showed that Suza 3 contained the bivalent Bt + CpTI bivalent insecticidal gene fragment synthesized in China.