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目的:用液质联用色谱法(LC-MS)分析石菖蒲去挥发油水提液的成分。方法:在Meth:H2O(5:95),pH10(氨水调),流速400μL/min,柱温室温(24℃)的条件下进行全扫描,根据文献选择一些已知化合物做选择离子监测(SIM),并对从选择离子监测结果中选出数个信号相对较强的可能化合物作二级质谱全扫描。结果:LC-MS共监测到9个已知化合物:4,5,8-trimethoxy-xan-thone-2-o-β-D-gluco-pyranosy(1→2)-o-β-D-gdactopyranoside(Ⅰ)、3β,22α,24,29-tetrahydroxyole-12-en-3-o{--β-D-arabinosy-(1→3)}-β-D-arabinopyranoside(Ⅱ)、Luteolin-6,8-C-diglucoside(Ⅲ)、1β,2α,3β,19α-tetrahydroxyurs-12-en-28-oic acid-28-o{--β-glu-copyranosil(1→2)}-β-glactopyranoside(Ⅳ)、菖蒲碱乙(Ⅴ)、高良姜素(Ⅵ)、细辛醛(Ⅶ)、1,2-dimethoxy-4-(E-3’-methyloxiranyl)benzene(Ⅷ)、菖蒲碱甲(Ⅸ)。结论:石菖蒲去油水提液9个成分中,可能包含有治疗癫痫的有效成分。
Objective: To analyze the constituents of the aqueous extract of Acorus calamus by liquid chromatography-mass spectrometry (LC-MS). Methods: Full scan was performed under the conditions of Meth: H2O (5:95), pH10 (NH3-H2O), flow rate of 400 μL / min and column temperature of 24 ℃. Some known compounds were selected according to the literature for SIM ), And from the selected ion monitoring results selected several relatively strong potential compounds for the second-level MS full scan. Results: Nine known compounds were detected by LC-MS: 4,5,8-trimethoxy-xan-thone-2-o-β-D-gluco- pyranosy (1 → 2) -o-β-D-gdactopyranoside (Ⅰ), 3β, 22α, 24,29-tetrahydroxyole-12-en-3-o {β-D-arabinosy- (1 → 3)} - β-D-arabinopyranoside 8-C-diglucoside (III), 1β, 2α, 3β, 19α-tetrahydroxyurs- 12-en- 28-oic acid-28-o {- β-glu-copyranosil (1- → 2)} - β-glactopyranoside (Ⅵ), ascorbic acid (Ⅵ), asarum (Ⅶ), 1,2-dimethoxy-4- (E-3’-methyloxiranyl) benzene ). Conclusion: Acorus gramineus oil extract of the 9 components, may contain active ingredients for the treatment of epilepsy.