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目的 :观察灵龙颗粒剂 (简称LLE)抗动物移植性肿瘤和裸鼠人肺癌与体外抑瘤作用及其对荷瘤小鼠免疫功能的影响。方法 :体内抗肿瘤实验采用腋下接种肿瘤细胞及实体瘤称重的方法 ;腹腔接种瘤液观察荷瘤动物存活率 ;裸鼠腋下接种人肺腺癌及实体瘤称重 ;体外抗肿瘤实验采用直接观察肿瘤细胞增殖的MTT法 ;2 ,4 二硝基氟苯诱发的DTH反应采用小鼠耳片差重法 ;淋巴细胞转化实验采用MTT法检测 ;TNF α活性检测采用L92 9细胞杀伤实验。结果 :LLE(13.1,17.5 ,35 .0 g生药·kg-1)对移植性动物肿瘤S180 及H2 2 的抑瘤率分别为 4 7.7% ,6 6 .1% ,82 .0 %及 6 7.0 % ,73.3% ,76 .4 % ;使荷EAC肿瘤小鼠的生存期延长 ;LLE(17.5 ,35 .0g生药·kg-1)对裸鼠人肺腺癌有较强的抑制作用 ,抑瘤率为 6 0 .5 %和 77.8% ;该药还显示一定的体外抑瘤作用 ;LLE对荷S180 及H2 2 小鼠的DTH反应有增强作用 ;对淋巴细胞转化有明显促进作用 ;LLE可有效增加荷瘤小鼠腹腔巨噬细胞TNF α的产生。结论 :igLLE对荷瘤小鼠具有显著的抗肿瘤作用 ,且能拮抗荷瘤所致的免疫功能的抑制。
Objective : To observe the anti-tumor effect of Linglong granules (LLE) against animal transplanted tumors and human lung cancer in nude mice and its effect on immune function of tumor-bearing mice. Methods: In vivo anti-tumor experiments were carried out by inoculating tumor cells and weighing solid tumors in the armpits; intraperitoneal inoculation of tumor fluid was used to observe the survival rate of tumor-bearing animals; nude mice were vaccinated subcutaneously with human lung adenocarcinoma and solid tumors; in vitro anti-tumor experiments The MTT method for direct observation of tumor cell proliferation was used; the DTH reaction induced by dinitrofluorobenzene (2,4 dinitrofluorobenzene) was performed using the mouse ear differential method; the lymphocyte transformation experiment was performed by MTT assay; the TNFα activity was detected by the L92 9 cell killing experiment. . Results: The tumor inhibition rates of LLE (13.1, 17.5, 35.0 g crude drug kg-1) in transplanted animal tumors S180 and H2 2 were 4 7.7%, 6 6.1 %, 82.0 % and 6 7.0, respectively. %, 73.3%, 76.4%; prolonged survival of EAC tumor mice; LLE (17.5, 35. 0g crude drug kg-1) has strong inhibitory effect on human lung adenocarcinoma in nude mice. The rate was 60.5% and 77.8%; the drug also showed certain anti-tumor effect in vitro; LLE enhanced the DTH response in S180 and H2 2 mice; it significantly promoted lymphocyte transformation; LLE was effective Increased TNF α production in peritoneal macrophages of tumor-bearing mice. Conclusion: igLLE has a significant anti-tumor effect on tumor-bearing mice and can antagonize the inhibition of tumor-induced immune function.