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目的:筛选前列腺癌转移相关基因,为研究前列腺癌的转移分子标志奠定基础。方法:将DU145细胞接种于裸鼠前列腺腹叶,5周后分别从原发灶和肺转移灶分离肿瘤细胞,利用cDNA芯片技术检测2种细胞中差异表达基因。结果:成功建立了前列腺癌细胞系原位移植自发转移裸鼠模型,经多轮筛选获得了前列腺癌原位及肺转移细胞。通过基因芯片筛选获得284条差异表达基因,基因功能涉及细胞信号转导、细胞周期、细胞凋亡、转录、黏附和代谢等。结论:基因芯片高通量筛选提示200余条基因中的某些基因可能与人前列腺癌转移有关。本研究为前列腺癌转移分子机制提供参考。
Objective: To screen the genes related to the metastasis of prostate cancer and lay a foundation for the study of molecular markers of metastasis in prostate cancer. Methods: DU145 cells were inoculated on the ventral lobe of the prostate of nude mice. Five weeks later, tumor cells were isolated from the primary and metastatic lung. The differentially expressed genes in the two cell lines were detected by cDNA microarray. Results: The nude mouse model of orthotopic transplantation of prostate cancer cell line was successfully established and the in situ and lung metastatic cells of prostate cancer were obtained by multiple rounds of screening. A total of 284 differentially expressed genes were obtained by gene chip screening. Gene functions involved in cell signal transduction, cell cycle, apoptosis, transcription, adhesion and metabolism. Conclusion: Gene chip high-throughput screening suggests that some genes of more than 200 genes may be involved in the metastasis of human prostate cancer. This study provides a reference for the molecular mechanism of prostate cancer metastasis.