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[目的]应用HPLC法测定阿维菌素-β-环糊精包合物的包封率。[方法]以外标法为基础,以Diamonsil?C18为色谱柱,流动相为甲醇-水(体积比90∶10),检测波长为245 nm,流速为1.0 mL/min,柱温为室温,进行HPLC分析。[结果]在0～400 mg/L的质量浓度范围内建立阿维菌素标准曲线,线性相关系数0.999 8,精密度的变异系数在0.63%～0.84%之间,回收率为96.4%～98.9%,回收率的变异系数在0.27%～0.83%之间。[结论]这一分析检测方法可靠有效。
[Objective] To determine the entrapment efficiency of abamectin-β-cyclodextrin inclusion by HPLC. [Method] On the basis of external standard method, Diamonsil C18 was used as the mobile phase. The mobile phase consisted of methanol and water (volume ratio 90:10). The detection wavelength was 245 nm, the flow rate was 1.0 mL / min, HPLC analysis. [Result] The standard curve of abamectin was established in the concentration range of 0 ~ 400 mg / L, the linear correlation coefficient was 0.999 8, the coefficient of variation of precision was between 0.63% ~ 0.84% and the recovery rate was 96.4% ~ 98.9 %, The coefficient of variation of recovery was between 0.27% ~ 0.83%. [Conclusion] This method is reliable and effective.