目的:以Con A诱导小鼠免疫性肝损伤模型,研究狭基线纹香茶菜对免疫肝损伤小鼠TNF-αmRNA和蛋白表达的影响,以探讨其抗免疫肝损伤的作用机理。方法:小鼠尾静脉注射ConA 25mg/kg制备免疫肝损伤模型,灌胃给予狭基线纹香茶菜水提部位7d后,不同时间点采取肝组织,提取总RNA,用反转录聚合酶链式反应(RT-PCR)对肝脏TNF-αmRNA进行半定量测定,免疫组化法检测肝组织TNF-α蛋白的表达。结果:与正常组相比,模型组小鼠肝组织4h TNF-αmRNA和蛋白表达显著增强,至8h仍保持较高的表达。阳性药PDTC和狭基线纹香茶菜水提部位大剂量组TNF-αmRNA和蛋白表达显著低于模型组(起效剂量9.2g/kg)。结论:ConA诱导损伤后,肝组织中TNF-αmRNA和蛋白表达明显增强,狭基线纹香茶菜组可通过降低损伤肝组织TNF-αmRNA和蛋白的表达,而对免疫肝损伤小鼠起保护作用。 OBJECTIVE: To study the effect of Conch-induced Acute Hepatic Injury in mice on the expression of TNF-αmRNA and protein in mice with immune-induced liver injury and to explore the mechanism of its anti-immune liver injury. Methods: The model of immunocompromised liver injury was induced by injection of ConA 25mg / kg into the caudal vein of mice. Gavage was given to the water extract of Syringa obtusiloba 7 days after oral administration. The liver tissue was taken at different time points to extract total RNA. Reverse transcriptase polymerase chain reaction The liver TNF-αmRNA was semi-quantitatively determined by RT-PCR and the expression of TNF-α protein in liver tissue was detected by immunohistochemistry. Results: Compared with the normal group, the expression of TNF-αmRNA and protein in liver of model group increased significantly at 4h, and maintained high level at 8h. The positive expression of TNF-αmRNA and protein in the positive drug PDTC and the narrow-basking Xiangchacao water extract were significantly lower than those in the untreated group (the starting dose was 9.2g / kg). CONCLUSION: The expression of TNF-αmRNA and protein in liver tissue after ConA-induced injury was significantly increased. The narrow base line Xiangchacao group could protect the mice with immunocompromised liver injury by decreasing the expression of TNF-αmRNA and protein in injured liver tissue .