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目的:探讨心梗后进行4周有氧运动干预对大鼠心脏交感神经标志物TH,促炎因子TNF-α与氧化应激水平的关系。方法:选取雄性SD大鼠36只,随机分为假手术对照(SC)组,心肌梗死(MI)组,心梗+持续有氧运动(ME)组,每组12只。MI组采用心脏左冠状动脉前降支(LAD)结扎法,建立MI模型。SC组大鼠实施假手术,ME组大鼠在MI手术后1周进行4周跑台运动。运动以10 m/min速度开始,运动5 min后,以3 m/min的速度递增至16 m/min。运动总时间均为60 min,5 d/周×4周。训练结束后测定各组大鼠心电图变化。之后开胸摘取心脏,进行组织学制片。免疫组化法观察分析左心室交感神经标记物TH及促炎因子TNF-α表达。Western Blot法检测TH及TNF-α蛋白含量。DHE法检测心肌超氧阴离子水平。结果:与SC组相比,MI组大鼠左心室中可见大量TH阳性神经纤维(P<0.01),其蛋白表达显著增加(P<0.01);同时TNF-α蛋白表达及超氧阴离子水平显著升高(均为P<0.01)。与MI组比较,ME组左心室TH、TNF-α蛋白表达及超氧阴离子水平均显著下降(分别为P<0.05,P<0.01,P<0.01);且发现左心室TH表达与ROS水平呈显著正相关(R=0.53,P<0.05),而TH与TNF-α相关性不显著(R=0.41,P=0.11)。结论:心梗后早期进行4周持续有氧运动干预可抑制心梗大鼠心脏交感神经标志物TH,其可能与抑制心肌氧化应激水平关系密切,与炎性细胞因子TNF-α水平关系不显著。
Objective: To investigate the relationship between cardiorespiratory sympathetic nerve injury (TH), proinflammatory cytokines (TNF-α) and oxidative stress in rats after cardio-infarction for 4 weeks. Methods: Thirty - six male Sprague - Dawley rats were randomly divided into 4 groups: sham operation control group (MI), myocardial infarction group (MI) and myocardial infarction + continuous aerobic exercise group (ME) The MI group was established by ligation of left anterior descending coronary artery (LAD) in MI group. Rats in SC group were sham operated. Rats in ME group were given treadmill exercise for 4 weeks 1 week after MI operation. The exercise started at a speed of 10 m / min and then increased to 16 m / min at a speed of 3 m / min after 5 min of exercise. Total exercise time was 60 min, 5 d / week × 4 weeks. The changes of electrocardiogram of rats in each group were measured after training. After thoracotomy remove the heart for histological preparation. Immunohistochemistry was used to observe the expression of TH and pro-inflammatory cytokine TNF-α in left ventricle. The contents of TH and TNF-α were detected by Western Blot. DHE method to detect myocardial superoxide anion level. Results: Compared with SC group, a large number of TH-positive nerve fibers were found in the left ventricle of rats in MI group (P <0.01), and the protein expression was significantly increased (P <0.01). At the same time, TNF-α protein expression and superoxide anion level were significantly (All P <0.01). Compared with MI group, the levels of TH, TNF-α and superoxide anion in left ventricular were significantly decreased in ME group (P <0.05, P <0.01, P <0.01, respectively) (R = 0.53, P <0.05), while the correlation between TH and TNF-α was not significant (R = 0.41, P = 0.11). CONCLUSION: Continuous cardiopulmonary intervention at 4 weeks after myocardial infarction can inhibit the cardiac sympathetic nerve TH, which may be related to the inhibition of myocardial oxidative stress, but not to the level of inflammatory cytokines TNF-α Significant.