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将松下牌微波炉（microwave oven，MWO）应用于20例人体尸检材料（心、脑）的链霉菌素一生物素免疫组化染色（LSAB法），检测了10种心、脑组织抗原．MWO应用于尸检材料免疫组化染色时的使用要点为（1）抗原的修复：切片置于0.01M枸椽酸盐缓冲液（PH6．0）中，DEF档（210W），10min，温度92－98℃；（2）滴加正常血清、一抗、二抗及SP复合物后，于LOW档（70W），5min，温度20～37℃,其它步骤与标准方法一致．结果表明：MWO的应用有助于封闭抗原的修复，提高阳性染色程度，降低背景着色，缩短染色时间．
Microwave oven (MWO) was applied to streptomycin-biotin immunohistochemical staining (LSAB method) in 20 human autopsy materials (heart and brain) to detect 10 kinds of heart and brain tissue antigens. MWO used in autopsy material immunohistochemical staining points for the use of (1) antigen repair: slice placed in 0.01M citrate buffer (PH6.0), DEF file (210W), 10min, temperature 92- 98 ° C; (2) After the addition of normal serum, primary antibody, secondary antibody and SP complex, the temperature is 20 ~ 37 ℃ in LOW file (70W) for 5min. The results showed that the application of MWO was helpful for the repair of the closed antigen, increasing the positive staining, reducing the background coloring and shortening the staining time.