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本文报道利用依主梨树茎尖、侧芽进行离体培养的实验过程.春、秋季取成年依主梨树一年生枝条的顶芽或侧芽作实验材料,其中秋季芽先用赤霉素打破休眠,经剥离、消毒后接种于诱导生长和分化的培养基上,生长约一个月后转入增殖培养基,40天后将增殖后伸长的芽条用于生根,其余小芽则继续增殖;生根的梨苗则进行移栽.通过实验,MS/2(大量元素)+MS(微量元素)+铁盐+有机成分(简称培养基A),附加6-BA 2mg/L、NAA 0.2mg/L、GA_3 1～3mg/L适于芽生长和分化;而附加6-BA 1.5mg/L、NAA 0.2mg/L、QA_3 2mg/L是优良增殖培养基;附加IBA 10mg/L处理8天的二步生根法较好;生根的试管苗已移栽定植成活.
In this paper, the experimental process of in vitro culture using the stem tips and lateral buds of the main pear tree was reported.The top buds or lateral buds of the annual branches of the main pear tree were taken as the experimental materials in spring and autumn, After stripping and sterilizing, the cells were inoculated on the medium for inducing growth and differentiation, transferred to proliferation medium after about one month of growth, and elongated buds were used for rooting after 40 days, and the remaining small buds continued to proliferate; And the transplanting of pear seedlings was carried out.Adding 6-BA 2mg / L and NAA 0.2mg / L, MS / 2 (mass element) + MS (trace element) + iron salt + organic component GA3 was suitable for bud growth and differentiation. GA6 supplemented with 6-BA 1.5mg / L, NAA 0.2mg / L and QA-32mg / L were good proliferation medium; Rooting method is better; rooted test tube seedlings have transplanted colonization survival.