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目的研究凝血酶、TNF－α.IFN-γ等对U937细胞表达uPARmRNA的影响，观察凝血系统、炎症介质与纤溶功能变化间的关系。方法建立并应用逆转录PCR（RT－PCR）法测定培养U937细胞的uPARmRNA。结果1．经测定批间变异系数（CV间）确认了RT－PCR法测定uPARmRNA的稳定性；2以不同浓度凝血酶刺激24h，对U937细胞表达uPARmRNA有抑制和促进两种不同的效应：TNF－α、IFN－γ、ATRA和As2O3都能促进uPARmRNA表达，但剂量-效应关系并不相同。结论与凝血和炎症反应相关的因子及某些药物以不同方式和程度影响细胞表达uPARmRNA，说明它们的作用机制和意义并不相同。
Objective To study the effects of thrombin, TNF-α, IFN-γ on the expression of uPAR mRNA in U937 cells and to observe the relationship between coagulation system, inflammatory mediators and changes of fibrinolytic function. Methods The uPAR mRNA of U937 cells was established by reverse transcription PCR (RT-PCR). Results 1. The stability of uPAR mRNA was confirmed by RT-PCR after the inter-assay coefficient of variation (CV) was confirmed.2 The uPAR mRNA expression of U937 cells was inhibited and promoted by different concentrations of thrombin for 24 h: TNF-α, Both IFN-γ, ATRA and As2O3 promoted uPAR mRNA expression, but the dose-response relationship was not the same. Conclusion The factors related to coagulation and inflammatory reaction and some drugs affect the expression of uPAR mRNA in different ways and in different degrees, indicating that their mechanism of action and significance are not the same.