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目的 通过观察肝素对人涎腺腺样囊性癌肺高转移细胞株 (ACC -M )细胞在裸鼠肺、肝组织的粘附影响 ,证实其对腺样囊性癌肺高转移细胞肺粘附的抑制作用。方法 裸鼠腹腔注射肝素后 ,经尾静脉注入氚标胸腺嘧啶核苷 (3 HTdR)标记的ACC -M细胞 (3 H -ACC -M ) ;分别检测细胞注射后 2h、6h、18h肺、肝组织单位重量的每分钟同位素脉冲数 (CPM )。结果 单位重量肺组织每分钟放射性计数CPM值 :空白对照组 2h、6h、18h分别为 1875 .75± 11.5 3、164 4.5 0± 2 7.2 0和 14 66.5 0± 12 5 .95 ;2 0 0单位肝素组 2h、6h、18h分别为 912 .0 0± 118.5 5、918.0 0± 44 .0 6和 766.60± 77.66。同一时间肝素组肺组织CPM值明显低于对照组 ,有显著性差异 (P <0 .0 0 1)。单位重量肝组织CPM值在空白对照组和肝素组的相同时间点无显著性差异 (P >0 .0 5 )。结论 肝素腹腔注射后可明显降低肺组织内ACC -M细胞对血管内皮细胞粘附数量 ,但对相同时间肝组织内ACC -M细胞粘附数量无显著影响 ,提示肝素具有抑制腺样囊性癌肺转移的作用
Objective To observe the effect of heparin on adhesion of human lung salivary adenoid cystic carcinoma cell line with high metastasis (ACC-M) in lung and liver tissues of nude mice and to confirm the effect of heparin on the lung adhesion of adenoid cystic carcinoma Attached to the inhibition. Methods Heparin was injected intraperitoneally into nude mice, and 3-HTdR-labeled ACC-M cells (3 H -ACC-M) were injected into the tail vein of the nude mice. The lung and liver were harvested at 2 hours, 6 hours and 18 hours after injection Tissue unit weight per minute isotope pulse (CPM). Results Per unit weight of lung tissue radioactivity counts per minute CPM value: blank control group 2h, 6h, 18h were 1875.75 ± 11.5 3,164 4.5 0 ± 2 7.2 0 and 14 66.5 0 ± 125.595; 200 units Heparin group 2h, 6h, 18h were 912.0 ± 118.5 5,918.0 ± 44.0 6 and 766.60 ± 77.66. At the same time, CPM in lung tissue of heparin group was significantly lower than that of control group (P <0.01). Per unit weight of liver tissue CPM value in the blank control group and heparin group at the same time point no significant difference (P> 0.05). Conclusion Intraperitoneal injection of heparin can significantly reduce the adhesion of ACC-M cells to vascular endothelial cells in the lung tissue, but no significant effect on the number of ACC-M cells in the liver at the same time, suggesting that heparin can inhibit adenoid cystic carcinoma The role of lung metastasis