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OBJECTIVE:To investigate the possible antinoci-ceptive effects of Salvia(S.)miltiorrhiza Bunge and its single components in monosodium urate(MSU)-induced pain model in mice and lipopolysac-charide(LPS)-induced inflammation model in RAW264.7 cells.METHODS:Pretreatment of S.miltiorrhiza Bunge extract(from 1 to 50μg/mL)concentration-de-pendently attenuated LPS-induced nitric oxide(NO)release.The extract of S.miltiorrhiza Bunge(50 or 100 mg/kg)also caused reversals of de-creased threshold for pain in the MSU-treated group as measured by Von-Frey test.Furthermore,we assessed the antinociceptive and anti-inflamma-tory properties of the active single components from S.miltiorrhiza Bunge such as 15,16-dihydro-tanshinoneⅠ,tanshinoneⅠ,cryptotanshinone,miltirone,tanshinoneⅡA,and salvianolic acid B.Some of them showed an anti-inflammatory effect in LPS-induced NO release model and an antinoci-ceptive effect in MSU-treated pain model.RESULTS:Our results suggest that S.miltiorrhiza Bunge extract may exert anti-inflammatory effect by reducing LPS-induced NO release and an antino-ciceptive property in MSU-treated pain model.Es-pecially,tanshinoneⅡA,miltirone,cryptotanshi-none,and 15,16-dihydrotanshinoneⅠ,not only ap-pear to be responsible for LPS-induced NO release induced by S.miltiorrhiza Bunge,but also in the production of S.miltiorrhiza Bunge extract-in-duced antinociception in MSU-treated pain model.CONCLUSION:Therefore,the analgesic and anti-in-flammatory property of S.miltiorrhiza Bunge indi-cate it as a therapeutic potential candidate for the treatment of pain and inflammation.