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从腹泻患儿新鲜粪便内,浓集、纯化贾第虫包囊。用生理盐水制成悬液,感染长爪沙鼠(Meriones unguiculatus)乳鼠。接种后第8d将受染鼠处死。用无菌术从上段小肠分离滋养体,接种于改良TYI-S-33培养基内,于37℃培养。培养后第14d,生长旺盛的虫体在培养管壁内面形成密集的细胞单层。生长高峰期和倍增时间分别在培养后的第120和15±2h。各批虫体经液氮冷冻复苏后,虫体复活率为53～80％。复苏虫体生长良好。本纯培养已维持9月余,传代100多次。
From diarrhea in children with fresh faeces, concentration, purification of Giardia cyst. Suspensions were made with normal saline and infected Meriones unguiculatus neonatal rats. The infected mice were sacrificed on the 8th day after inoculation. The trophozoites were isolated from the upper intestine by aseptomy and inoculated into modified TYI-S-33 medium and cultured at 37 ° C. On the 14th day after culture, the well-growing parasites formed dense cell monolayers on the inner surface of the culture tube. Growth peak and doubling time were 120 and 15 ± 2 h after culture respectively. After each batch of insect body liquid nitrogen frozen recovery, the resurrection rate of 53% to 80%. Resuscitation parasites grow well. The pure culture has been maintained for more than 9 months, more than 100 times.