论文部分内容阅读
目的：探讨大肠癌的发病机理及早期诊断的酶标志物。方法：采用酶学方法测定大肠癌及近心端和远心端癌旁组织中鸟氨酸脱羧酶（ＯＤＣ）、γ－谷氨酰转肽酶（γ－ＧＴ）、乳酸脱氢酶（ＬＤＨ）活性及ＬＤＨ同工酶谱。结果：（１）大肠癌组织中ＯＤＣ、γ－ＧＴ及ＬＤＨ活性均显著高于癌旁正常组织；（２）大肠癌组织中ＬＤＨ５同工酶及ＬＤＨ４＋ＬＤＨ５／ＬＤＨ１＋ＬＤＨ２的比值也显著升高，ＬＤＨ同工酶谱向Ｍ型转移。结论：肠组织中ＯＤＣ、γ－ＧＴ、ＬＤＨ活性升高，ＬＤＨ同工酶谱向Ｍ型转移与大肠癌的形成有关，提示ＯＤＣ、γ－ＧＴ及ＬＤＨ可能是大肠癌的组织酶标志物
Objective: To investigate the pathogenesis of colorectal cancer and early diagnosis of enzyme markers. METHODS: Ornithine decarboxylase (ODC), γ-glutamyl transpeptidase (γ-GT), and lactate dehydrogenase (LDH) were detected by enzymatic method in colorectal cancer tissues and near- and distal-adjacent tissues. ) Activity and LDH isozyme patterns. RESULTS: (1) The activity of ODC, γ-GT and LDH in colorectal cancer tissues were significantly higher than those adjacent to normal tissues; (2) The ratios of LDH5 isoenzymes and LDH4+LDH5/LDH1+LDH2 in colorectal cancer tissues were also significantly increased. The zymogram is transferred to M type. Conclusion: The activities of ODC, γ-GT, and LDH in the intestine are increased. The transfer of LDH isoenzyme pattern to M-type is related to the formation of colorectal cancer, suggesting that ODC, γ-GT and LDH may be the tissue enzyme markers of colorectal cancer.