Mechanical construction will put influence on the biological characters of reclaimed soils, as well as the soil quality. In order to explore the changing rule of soil microbial quantity and respiratory capacity under different con-struction machineries and rolling times, and find the optimal processing conditions, an experiment was set up and a simulation experimental area was chosen, in which we simulated the main types of reclamation in coal mine area. After 2 years’ natural aging, we collected surface soil samples (0–20 cm) that can be used for experimental analysis. The result shows that changing rules of soil biological factors are different with different construction machineries, and soil properties are closest to the normal soil when adopting the combination of ‘‘crawler dozer 9 5 compaction times’’ and ‘‘dump truck 9 3 compaction times’’, which shows that the soil quality is better under this condition.