By means of polymerase chain reaction(PCR) technique,direct smear fluorescence microscopy and bacterial culture,the sputa and purulent secretion of 122 TB patients were examined to detect mycobacterium tuberculosis. By means of polymerase chain reaction (PCR) technique, direct smear fluorescence microscopy and bacterial culture, the sputa and purulent secretion of 122 TB patients were examined to detect mycobacterium tuberculosis.