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目的探讨葡萄籽提取物原花青素(GSPE)对三氧化二砷(As2O3)所致雄性小鼠肝脏毒性的干预效果及其抗氧化损伤机制在其中的作用。方法应用2×2析因设计按体重分层将40只昆明种雄性小鼠随机分为对照组(0.9%生理盐水)、As2O3组(4.0 mg/kg)、GSPE组(400 mg/kg)和As2O3(4.0 mg/kg)+GSPE(400 mg/kg)组,每组10只,连续灌胃5周;称量小鼠体重、肝重并计算肝脏系数;测定肝组织天冬氨酸转氨酶(AST)与丙氨酸转氨酶(ALT)活力、丙二醛(MDA)含量、谷胱甘肽(GSH)含量、超氧化物歧化酶(SOD)活力、总抗氧化能力(T-AOC)水平;GSPE与As2O3的交互作用采用析因设计方差分析。结果与对照组相比,As2O3组体重、GSH含量和T-AOC水平降低(P<0.05),肝重、肝脏系数、AST与ALT活力、MDA含量升高(P<0.05)。析因分析显示As2O3与GSPE两种因素对上述指标的交互作用均有统计学意义(P<0.05)。与As2O3组相比,As2O3+GSPE组体重、GSH含量、T-AOC水平升高(P<0.05),肝重、肝脏系数、AST与ALT活力、MDA含量降低(P<0.05)。结论 GSPE可能通过抗氧化损伤机制拮抗砷致雄性小鼠的肝脏毒性。
Objective To investigate the intervention effect of grape seed extract proanthocyanidins (GSPE) on hepatotoxicity in male mice induced by As 2 O 3 and its anti-oxidative mechanism. Methods 40 Kunming male mice were randomly divided into control group (0.9% saline), As2O3 group (4.0 mg / kg), GSPE group (400 mg / kg) 10 rats in each group were fed with As2O3 (4.0 mg / kg) and GSPE (400 mg / kg) for 5 weeks. The body weight and liver weight of mice were weighed and the liver coefficient was calculated. The levels of aspartate aminotransferase AST, ALT, MDA, GSH, T-AOC, GSPE and As2O3 interaction using factorial design analysis of variance. Results Compared with the control group, the body weight, GSH level and T-AOC level in As2O3 group decreased (P <0.05). The liver weight, liver coefficient, AST and ALT activity and MDA content increased (P <0.05). Factor analysis showed that As2O3 and GSPE two factors on the above indicators of interaction were statistically significant (P <0.05). Compared with As2O3 group, body weight, GSH content and T-AOC level in As2O3 + GSPE group increased (P <0.05), liver weight, liver coefficient, AST and ALT activity and MDA content decreased (P <0.05). Conclusion GSPE may antagonize the liver toxicity of arsenic-induced male mice through the mechanism of antioxidant damage.