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由于猕猴桃种子繁殖的后代发生分离,无法利用种子来繁殖优良株系。同时初选优良单株枝条也有限,用枝接和扦插法一时又难以繁殖出大批苗木。针对这个问题,我们于1980年开始,试图应用组织培养技术繁殖猕猴桃的优良单株。一、愈伤组织的诱导和芽的分化我们于1980年2月剪取中华猕猴桃优良单株的一年生枝条,长约10厘米,放在1:30新洁尔灭水稀释液中表面消毒20分钟,再用无菌水冲洗5遍,剥去皮层,
Due to the separation of offspring of kiwifruit seeds, good lines can not be breed using seeds. At the same time primaries fine single branches is also limited, with sticks and cuttings temporarily difficult to breed a large number of seedlings. In response to this problem, we started in 1980, trying to apply tissue culture technology to reproduce the fine lines of kiwifruit. I. Callus Induction and Bud Differentiation We cut the annual shoots of excellent Chinese kiwifruit plants in February 1980 for about 10 cm in length and sterilized them on a 1:30 dilution of Broth in water for 20 minutes, Sterile water rinse 5 times, stripped the cortex,