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目的:探讨人参皂苷Rg1对尿蛋白诱导肾小管上皮细胞损伤后肾小管上皮细胞的再生作用及机制。方法:体外培养的HK2细胞,予不同浓度尿蛋白刺激,MTT法、LDH比色法观察细胞的成活及细胞膜稳定性;以人参皂苷Rg1进行干预,肝细胞生长因子(HGF)为阳性对照,用MTT法检测细胞存活率,western blot和实时定量RT-PCR检测增殖细胞核抗原(PCNA)基因及蛋白水平。结果:一定浓度的尿蛋白能够显著减少HK2细胞存活率、细胞膜稳定性(P<0.05),且存在浓度效应;浓度为3mg/m L的尿蛋白刺激下的HK2细胞PCNA蛋白和PCNA m RNA表达显著降低(P<0.05);而人参皂苷Rg1能提高尿蛋白损伤的HK2细胞存活率,且上调了PCNA蛋白的表达(P<0.05),与HGF比较无差异。结论:超载尿蛋白致体外培养的肾小管上皮细胞存活率降低,人参皂苷Rg1可提高尿蛋白损伤的肾小管上皮细胞存活率,促进细胞增殖。
Objective: To investigate the effect and mechanism of ginsenoside Rg1 on renal tubular epithelial cells after urinary protein-induced tubular epithelial cell injury. Methods: HK2 cells cultured in vitro were stimulated with different concentrations of urinary protein. MTT assay and LDH colorimetric assay were used to observe cell viability and cell membrane stability. Interfered with ginsenoside Rg1, hepatocyte growth factor (HGF) was used as positive control Cell viability was detected by MTT assay. PCNA gene and protein levels were detected by western blot and real-time quantitative RT-PCR. Results: A certain concentration of urine protein could significantly reduce the viability of HK2 cells and the stability of cell membrane (P <0.05), and there was a concentration effect. The expression of PCNA protein and PCNA mRNA in HK2 cells stimulated with urinary protein at a concentration of 3 mg / m L (P <0.05). However, ginsenoside Rg1 could enhance the survival rate of HK2 cells induced by urinary protein and up-regulate the expression of PCNA protein (P <0.05), but no difference with HGF. CONCLUSION: The survival rate of tubular epithelial cells cultured in vitro induced by urinary protein is decreased. Ginsenoside Rg1 can enhance the renal tubular epithelial cell survival rate and promote cell proliferation induced by urinary protein.