论文部分内容阅读
目的:分析谷胱甘肽巯基转移酶(glutathione S-transferase,GST)在黏液性及非黏液性大肠癌组织中的表达情况,并探讨其临床意义。方法:选取2007年1月至2012年5月在上海交通大学附属新华医院肛肠外科行手术治疗的109例黏液性大肠癌标本和441例非黏液性大肠癌标本,运用免疫组化方法检测蛋白GST、nm23、细胞角蛋白7(cytokeratin 7,CK7)在黏液性大肠癌和非黏液性大肠癌组织中的表达情况,并探讨其临床意义。结果:GST和nm23在黏液性大肠癌组织中的阳性表达率分别为52.3%和57.8%,明显低于非黏液性大肠癌组织中的阳性率(64.7%,73.0%;均P<0.05),而CK7在黏液性大肠癌组织中的阳性表达率明显高于非黏液性大肠癌组织中的阳性率(27.5%vs 18.4%,P=0.033)。GST的表达与黏液性大肠癌的较低分化程度和较多淋巴结转移相关,nm23和CK7的表达与黏液性大肠癌的临床特征无明显相关性。结论:GST在黏液性和非黏液性大肠癌中存在异常表达,GST蛋白有可能成为检测黏液性大肠癌淋巴结转移和分化程度的生物学指标。
Objective: To analyze the expression of glutathione S-transferase (GST) in mucoid and non-mucous colorectal cancers and to investigate its clinical significance. Methods: From January 2007 to May 2012, 109 cases of mucinous colorectal cancer and 441 cases of non-mucinous colorectal cancer were surgically treated in the Department of Anorectal Surgery, Xinhua Hospital, Shanghai Jiao Tong University. Immunohistochemistry was used to detect the protein GST , Nm23, cytokeratin 7 (CK7) in mucinous colorectal cancer and non-mucinous colorectal cancer, and to explore its clinical significance. Results: The positive rates of GST and nm23 in mucinous colorectal cancer were 52.3% and 57.8%, respectively, which were significantly lower than those in non-mucinous colorectal carcinoma (64.7%, 73.0%, P <0.05) The positive rate of CK7 in mucinous colorectal cancer was significantly higher than that in non-mucinous colorectal cancer (27.5% vs 18.4%, P = 0.033). The expression of GST was correlated with the lower differentiation and lymph node metastasis in mucinous colorectal carcinoma. The expression of nm23 and CK7 had no significant correlation with the clinical features of mucinous colorectal carcinoma. CONCLUSIONS: GST is abnormally expressed in mucinous and non-mucoid colorectal cancers. GST protein may be a biological marker for detecting lymph node metastasis and differentiation in mucinous colorectal cancer.