论文部分内容阅读
目的:通过建立穹窿切断的动物模型来检测糖皮质激素的浓度和穹窿切断同时摘除肾上腺的动物模型检测海马神经元核受体-糖皮质激素受体(GR)的表达变化,以探讨GR参与下丘脑-垂体-肾上腺(HPA)轴调节的机制。方法:建立大鼠穹窿切断模型,采用ELISA方法检测穹窿切断0,4,7,10d血中糖皮质激素的浓度、建立大鼠穹窿切断同时摘除肾上腺7d的模型,采用免疫组织化学、免疫印迹方法检测海马神经元核受体GR表达变化的观察及定量检测。结果:穹窿切断7d后血中糖皮质激素的浓度升高,穹窿切断同时摘除肾上腺7d后,海马神经元GR表达升高。结论:海马核受体GR的表达变化可能与糖皮质激素的浓度呈负相关。
OBJECTIVE: To detect the changes of glucocorticoid receptor (GR) expression in hippocampal neurons by establishing the animal model of fornix to detect the concentration of glucocorticoids and to study the effects of GR on the expression of glucocorticoid receptor (GR) in hippocampal neurons. The mechanism of thalamic-pituitary-adrenal (HPA) axis modulation. Methods: The rat model of fornix was established. The concentration of glucocorticoid in the blood was detected by ELISA at 0, 4, 7 and 10 days after the fornix. The model of rat fornix was established and the adrenal was excised for 7 days. Immunohistochemistry and Western blot To detect the expression of nuclear receptor GR in hippocampal neurons and its quantitative detection. Results: The levels of glucocorticoid in the blood increased after the fornix was cut off for 7 days. The expression of GR in the hippocampal neurons increased after the fornix was cut off and the adrenal gland was excised for 7 days. Conclusion: The expression of nuclear receptor GR in hippocampus may be negatively correlated with the glucocorticoid concentration.